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品种反应性 主要应用 宿主 格式 抗体类型 Po, H, R, M, B WB, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1530 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Peripherin Antibody 背景信息 Peripherin is a 56-58 kDa type III intermediate filament protein (Portier et al. 1983), expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung's disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness. 产品信息 格式 Serum 控制 - Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.
演示 Rabbit polyclonal antisera, no preservatives. 应用 应用 This Anti-Peripherin Antibody is validated for use in IH, IH(P), WB for the detection of Peripherin. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Additional Research Applications
Immunohistochemistry:
A previous lot of this antibody was used at 1:100-1:200 dilution. It is suggested that the antibody be used on frozen sections fixed in acetone at -20°C. AB1530 will work on tissue fixed for one hour or less in fresh 4% paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections. See Cell & Tissue Research (1997) 288:11-23 & European J. Dermatology (1998) 8:339-342.
Electron Microscopy:
A previous lot of this antibody was used on Electron Microscopy.
Optimal working dilutions and protocols must be determined by end user.生物信息 免疫原品种 Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Peripherin. The antibody stains a ~57 kDa band cleanly and specifically and does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Strong staining on rat, mouse, human, pig and cow peripherin. Does not stain chicken, quail or other more distantly related species which appear to lack peripherin. 品种反应性 PigHumanRatMouseBovine Species Reactivity Note Strong staining on rat and human. Cross-reacts with mouse, pig, and bovine. Other species not tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006253
Entrez基因汇总 This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. 基因符号 - NEF4
- PRPH
- Peripherin
- PRPH1
- peripherin
纯化方法 Unpurified UniProt编号 - P41219
UniProt汇总 FUNCTION: SwissProt: P41219 # Class-III neuronal intermediate filament protein.
SIZE: 470 amino acids; 53651 Da
SIMILARITY: SwissProt: P41219 ## Belongs to the intermediate filament family.产品使用声明 质量保证 Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected peripherin on 10 μg of PC12 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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