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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Affinity Purified Monoclonal Antibody 描述 产品目录编号 05-500 品牌系列 Upstate
商名 - Upstate
描述 Anti-TAFII250 Antibody, clone 6B3 Alternate Names - EC 2.7.11.1
- TAF1 RNA polymerase II
- TBP-associated factor 1
- Transcription initiation factor TFIID subunit 1
- BALB/c 3T3 ts2 temperature sensitivity complementing
- Cell cycle gene 1 protein
- TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated<br />factor, 250kDa
- cell cycle, G1 phase defect
- dystonia 3
- TAFII-250
- TAF2A
- CCGS
- CCG1
- BA2R
- NSCL2
- BA2R
- NSCL2
背景信息 TATA-binding protein associated factor 250 (TAFII250) is the largest subunit of the transcription factor TFIID. Studies have shown that TAFII250 has serine/threonine domains that can autophosphorylate and transphosphorylate the large subunit of TFIIF. More recent studies have shown that TAFII250 is also a histone acetyltransferase, suggesting that transcriptional co-activators may also have a role in targeting acetylation activity to promoters. 产品信息 格式 Affinity Purified 控制 - Use 20 μg per lane for minigels.
- HeLa nuclear lysate, cat. no. 12-309
演示 Purified mouse monoclonal IgG1k in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide containing 30% glycerol 应用 应用 Detect TAFII250 using this Anti-TAFII250 Antibody, clone 6B3 validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 1 μg/ml of this lot detected TAFII250 from HeLa nuclear extract. Optimal working dilutions must be determined by the end user. 生物信息 免疫原品种 Full-length human TAFII250. 克隆 clone 6B3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes TAFII250, Mr 250kDa. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Human. Weak cross-reactivity with mouse and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - 6872
Entrez基因汇总 Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.
基因符号 - CCG1
- TAF1
- TAF1 RNA polymerase II
- TAF2A
- BA2R
- CCGS
纯化方法 Protein G purfied UniProt编号 - P21675
产品使用声明 质量保证 Routinely evaluated by western blot on HeLa nuclear extracts. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. For maximum recovery of product, centrifuge the vial prior to removing the cap 包装信息 数量 200 µg
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