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应用说明 品种 宿主细胞 蛋白靶 Calcium Flux Assays Human CHO (Chinese hamster ovary cells) D1 描述 产品目录编号 HTS102LT 商名 - ChemiBrite
描述 ChemiBrite™ D1 Dopamine Family Receptor Frozen Cells 背景信息 Millipore’s ChemiBrite cells co-express a GPCR along with a variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.
Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Dopamine exerts its action by binding to five distinct dopamine receptors, all of which belong to G protein-coupled receptor family (Missale et al. 1998). The D1 subtype is the most abundant dopamine receptor in the central nervous system. Activation of D1 receptor stimulates adenylyl cyclase, activates cyclic AMP-dependent protein kinases. It regulates neuronal growth and development, mediate some behavioral responses and modulate dopamine receptor D2-mediated events. Millipore’s cloned human D1 receptor-expressing ChemiBrite cells are made by transient transfection of CHO cells of optimized quantities of plasmids encoding the receptor, clytin and a promiscuous G protein to couple the receptor to the calcium signaling pathway and then cryopreserving the cells at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis of agonists, antagonists and modulators at the D1 receptor.产品信息 部件 - Vial contains 1 ml of mycoplasma-free cells
应用 应用 Human D1 GPCR frozen cells for Calcium Flux FLIPR Assays. 应用说明 Calcium Flux Assays 生物信息 品种 Human Entrez基因编号 - NM_000794
GPCR类别 A 宿主细胞 CHO (Chinese hamster ovary cells) 蛋白靶 D1 靶子系列 Dopamine 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
包装信息 数量 1 vial
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