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描述 概述 The K-LISA™ mTOR (Recombinant) Activity Kit (CBA104) is a sensitive, non-radioactive, ELISA-based assay for mTOR kinase activity. The kit is suitable for measuring the activity of purified or partially purified preparations of mTOR or mTOR that has been immunoprecipitated from crude cell lysates. The mTOR, Human, Recombinant, S. frugiperda supplied in the kit is an active fragment of mTOR (amino acids 1360-2549) purified from baculovirus-infected insect cells that can be used for in vitro screening of mTOR inhibitors. 产品目录编号 CBA104 品牌系列 Calbiochem®
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Guertin, D.A. and Abraham, R.T. 2007. Cancer Cell 12, 9.
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Ali, S.M., et al. 2005. J. Biol. Chem. 280, 19445.
Guertin, D.A., et al. 2005. Trends Mol. Med. 11, 353.
Sarbassov, D.D., et al. 2005. Science 307, 1098.
Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
Sarbassov, D.D., et al. 2004. Curr. Biol. 14, 1296.
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Sabatini, D.M., et al. 1994. Cell 78, 35.产品信息 形式 96 Tests 格式 96-well plate 试剂盒包含 Glutathione-Coated 96-Well Plate, mTOR Substrate, mTOR Inhibitor Control, mTOR Human Recombinant S. frugiperda, Anti-p70S6K-T389, 2X Kinase Assay Buffer, 20 mM ATP Solution, 100 mM DTT, Kinase Stop Solution, 10X Plate Wash Concentrate, Antibody Diluent, HRP-Antibody Conjugate, TMB Substrate, ELISA Stop Solution, Plate Sealers, and a user protocol. 产品使用声明 预定用途 The K-LISA™ mTOR (Recombinant) Activity Kit is suitable for measuring the kinase activity of purified or partially purified preparations of mTOR or mTOR that has been immunoprecipitated from crude cell lysates. The assay is useful for mTOR inhibitor screening or the characterization of mTOR activity. 储存和货运信息 货运代码 Multiple Storage Conditions
毒性 Regulatory Review
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 Multiple storage requirements
存储条件 Upon arrival store the Glutathione-Coated 96-Well Plate, the Kinase Stop Solution, the 2X Kinase Assay Buffer, and the Anti-p70S6K-pT389 at 4°C; store the mTOR Substrate, 100 mM DTT, 20 mM ATP Solution and mTOR, Human, Recombinant, S. frugiperda at -70°C; store the remaining components of the kit at -20°C.
Note: THE KIT IS PACKAGED IN 3 BOXES. TWO BOXES ARE SHIPPED ON DRY ICE; UPON ARRIVAL, ONE OF THESE IS STORED AT -20°C AND THE OTHER IS STORED AT -70°C. THE THIRD BOX CONTAINS 2X KINASE ASSAY BUFFER, IS SHIPPED ON BLUE ICE, AND IS STORED AT 4°C; DO NOT FREEZE THE 2X KINASE ASSAY BUFFER. DO NOT FREEZE/THAW THE ANTI-p70S6K-T389 ANTIBODY. STORE RECOMBINANT mTOR AT -70°C AND AVOID MULTIPLE FREEZE/THAW CYCLES-FOLLOWING INITIAL THAW, PROMPTLY ALIQUOT ANY UNUSED PROTEIN TO PRECHILLED MICROFUGE TUBES AND STORE AT -70°C.避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Glutathione-Coated 96-Well Plate, mTOR Substrate, mTOR Inhibitor Control, mTOR Human Recombinant S. frugiperda, Anti-p70S6K-T389, 2X Kinase Assay Buffer, 20 mM ATP Solution, 100 mM DTT, Kinase Stop Solution, 10X Plate Wash Concentrate, Antibody Diluent, HRP-Antibody Conjugate, TMB Substrate, ELISA Stop Solution, Plate Sealers, and a user protocol.
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