- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
检测方法 Fluorometric 描述 概述 The InnoZyme™ TACE Activity Kit is a specific and sensitive assay designed to measure human TACE activity in cell lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well Plate is pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an excitation wavelength of ~324 nm and emission wavelength of ~400 nm. The level of fluorescence is directly related to the enzyme activity.
• Highly specific: based on immunocapture of human TACE
• Highly sensitive and quantitative: assay range 5-100 ng/ml
• Convenient: 96-well format and non-radioactive detection
• Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors
产品目录编号 CBA042 品牌系列 Calbiochem®
同义词 α-Secretase Activity Kit, TNF-α Converting Enzyme Activity Kit, ADAM17 Activity Kit 应用数据 
The activity of increasing concentrations human recombinant TACE (Cat. No. PF133) was measured according to the Detailed Protocol above.
Human colorectal adenocarcinoma cells, DLD1 and HT-29 and human glioblastoma cells, T98G, were cultured in DMEM medium supplemented with 10% FCS and harvested at 80-90% confluency. Total cell lysates were prepared using CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) and the manufacturer's recommended protocol. TACE activity was measured according to the Detailed Protocol above. RFU is reported per mg protein.参考 参考 Kirkegaard, T., et al. 2004. Clin. Exp. Immunol. 135, 146.
Neumann, U., et al. 2004. Anal. Biochem. 328, 166.
Skovronsky, D.M., et al. 2001. J. Neurobiol. 49, 40.
Black, R.A., et al. 1997. Nature 385, 729.
Moss, M.L., et al. 1997. Nature 385, 733.
Selected Citations
DasGupta S., et al. 2009. Bioorg. Med. Chem. 17, 3604.
产品信息 部件 检测方法 Fluorometric 声明 Not available for sale in Germany. 形式 96 Tests 格式 96-well plate 试剂盒包含 Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol. 正控制 Human recombinant TACE 生物信息 化验范围 5-100 ng/ml as measured with purified recombinant human TACE 样品类型 Cells 产品使用声明 预定用途 The InnoZyme™ TACE Activity Kit is designed to measure human TACE activity in cell lysates and biological samples. The assay may also be used for screening enzyme inhibitors, but the end-user must supply purified enzyme for this application. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon receipt the unopened kit should be stored at -20°C or -70°C. All kit components, once opened, can be stored for up to 3 months under the following conditions:
Refrigerate (4°C):
Sample Buffer
Assay Buffer
Wash Buffer
Substrate
Anti-Human TACE-coated 96-well plate
Deep Freeze (-70°C):
Control*
*Note: Following initial use the Control should be dispensed into aliquots and stored at -70°C. Avoid freeze/thaw cycles.避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol.
上一件merck millipore产品：merck millipore,默克密理博,373385,Hh Signaling Antagonist VII, JK184 - CAS 315703-52-7 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,ABN351,Anti-LGI3 Antibody
相关产品
merck millipore,默克密理博,MABN1038,Anti-SBDS Antibody, clone EPR7820, rabbit monoclonal
merck millipore,默克密理博,MAB821P-K,Anti-Herpes Simplex Virus I&2, Clone 4F10.3
merck millipore,默克密理博,03-190,HDAC1 (Hist-tag) human recombinant
merck millipore,默克密理博,14-459M,Abl Protein, active, mouse, 250 µg
merck millipore,默克密理博,373385,Hh Signaling Antagonist VII, JK184 - CAS 315703-52-7 - Calbiochem
merck millipore,默克密理博,ABN351,Anti-LGI3 Antibody
merck millipore,默克密理博,203695,bpV(phen) - CAS 42494-73-5 - Calbiochem
merck millipore,默克密理博,382115,Histone Acetyl Transferase Inhibitor VII, CTK7A - Calbiochem
merck millipore,默克密理博,MABN721,Anti-PTK7 Antibody, clone 4F9
merck millipore,默克密理博,FCMAB423PE,Milli-Mark Anti-HDAC2-PE Antibody, clone 3F3