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检测方法 Fluorometric 描述 概述 A convenient assay for the chemotaxis cell migration. Cells migrate through a 8 μm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye (Excitation max.: ~485 nm; Emission max.: ~520 nm). 产品目录编号 CBA010 品牌系列 Calbiochem®
应用数据 
~50,000 human umbilical venous endothelial cells (HUVECs) were incubated in triplicate wells of the upper chamber and allowed to migrate toward medium with or without chemotactic components in the lower chamber for 4 h in a 6% CO2 incubator at 37°C. The cells were detached and labeled as indicated in the Detailed Protocol. Latrunculin A, a powerful inhibitor of actin polymerization, was added to an aliquot of cell suspension immediately prior to loading the cells in the wells.
~60,000 HT-1080 cells were incubated in triplicate wells of the upper chamber and allowed to migrate toward medium with or without serum for 3 h in a 6% CO2 incubator at 37°C. The cells were detached and labeled as indicated in the Detailed Protocol.参考 参考 Klinghoffer, R.A., et al. 1999. EMBO J. 18, 2459.
Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677.
Smilenov, L. B., et al. 1999. Science 286, 1172.
Burridge, K., et al. 1997. Trends Cell Biol. 7, 342.
Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719.
Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359.产品信息 检测方法 Fluorometric 形式 96 Tests 格式 96-well plate 试剂盒包含 Sterile 96-Well Migration Chamber, Culture Tray, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol. 应用 应用参考 Loberg, R.D., et al. 2006. Neoplasia 8, 578. 生物信息 化验时间 3-5 h 样品类型 Adherent cells 产品使用声明 预定用途 The Calbiochem® InnoCyte™ Cell Migration Assay is designed to study the effects of various drugs or agents on cell motility or for identifying chemoattractant molecules (chemotactic migration). The assay is not intended for leukocyte migration experiments, as the study of leukocytes requires membranes with a pore-size smaller than 8 µm. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C. 避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Sterile 96-Well Migration Chamber, Culture Tray, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
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