merck millipore,默克密理博,07-405,Anti-PRMT5 Antibody

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merck millipore,默克密理博,07-405,Anti-PRMT5 Antibody
产品名称：Anti-PRMT5 Antibody
产品型号：07-405
Anti-PRMT5 Antibody is a Rabbit Polyclonal Antibody for detection of PRMT5 also known as 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog & has bee More>>

merck millipore,默克密理博,07-405,Anti-PRMT5 Antibody

产品介绍

merck millipore,默克密理博,07-405,Anti-PRMT5 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-405
Replaces	MABE551
品牌系列	Upstate
商名	Upstate
描述	Anti-PRMT5 Antibody
概述	PRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.
Alternate Names	skb1 (S. pombe) homolog 72 kDa ICln-binding protein HMT1 hnRNP methyltransferase-like 5 Jak-binding protein 1 SKB1 homolog SKB1 homolog (S. pombe) Shk1 kinase-binding protein 1 homolog protein arginine methyltransferase 5
背景信息	Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.

产品信息
格式	Purified
控制	Jurkat cell lysate, HeLa cell lysate. Included Positive Antigen Control: Catalog # 12-303, Jurkat lysate. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示	Purified rabbit IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

应用
应用	Anti-PRMT5 Antibody is a Rabbit Polyclonal Antibody for detection of PRMT5 also known as 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog & has been validated in IP & WB.
主要应用	Western Blotting
应用说明	Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	PRMT5, Mr ~70 kDa
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_006109
基因符号	SKB1 PRMT5 HRMT1L5 IBP72 SKB1Hs JBP1
纯化方法	Protein A purfied
UniProt编号	O14744
UniProt汇总	FUNCTION: SwissProt: O14744 # Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. SIZE: 637 amino acids; 72684 Da SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Ubiquitous. PTM: Disulfide bonds and non-covalent association mediate homooligomers formation. SIMILARITY:SwissProt: O14744 ## Belongs to the protein arginine N-methyltransferase family.

产品使用声明
质量保证	Routinely evaluated by Western Blot in RIPA lysates from 3T3/A31, NIH 3T3,HeLa, WI-38, and Jurkat cells. Western Blot Analysis: 0.5-1 μg/mL of this lot detected PRMT5 in RIPA lysates from 3T3/A31, NIH 3T3, HeLa, WI-38, and Jurkat cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.