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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3594-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aquaporin 4 Antibody, CT Alternate Names - Mercurial-Insensitive Water Channel
背景信息 Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker. 产品信息 格式 Purified 控制 - Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Purified rabbit polyclonal lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). 应用 应用 Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user生物信息 免疫原品种 GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863). 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel). 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse, and rat. Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001650.4
Entrez基因汇总 This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. 基因符号 - MGC22454
- AQP4
- MIWC
- WCH4
- AQP-4
- HMIWC2
纯化方法 Immunoaffinity purified UniProt编号 - P55087
UniProt汇总 FUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
SIZE: 323 amino acids; 34830 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. ...产品使用声明 质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 200 µL
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