merck millipore,默克密理愽
  • merck millipore,默克密理博,MABE348,Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

  • merck millipore,默克密理博,MABE348,Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

    产品名称：Anti-phospho-RNAPII Antibody (Thr4), clone 1G7
    产品型号：MABE348
    Detect the phospho-RNAPII (Thr4) protein using this Anti-phospho-RNAPII (Thr4), clone 1G7 validated for use in wb, IP, ICC & ChIP.

    merck millipore,默克密理博,MABE348,Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

  • 产品介绍
  • merck millipore,默克密理博,MABE348,Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Yeast, H, MChIP, WB, ICCRPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABE348
    描述 Anti-phospho-RNAPII Antibody (Thr4), clone 1G7
    Alternate Names
    • RNA-directed RNA polymerase II subunit RPB1
    • DNA-directed RNA polymerase II subunit RPB1
    • RNA polymerase II subunit B1
    • DNA-directed RNA polymerase II subunit A
    • DNA-directed RNA polymerase III largest subunit
    背景信息 RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
    产品信息
    格式 Purified
    控制
    • Untreated and lambda phosphatase treated HeLa cell lysates.
    演示 Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Detect the phospho-RNAPII (Thr4) protein using this Anti-phospho-RNAPII (Thr4), clone 1G7 validated for use in wb, IP, ICC & ChIP.
    主要应用
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cell extracts (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

    Immunofluorescence Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cells (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

    Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Thr4) in stably transfected Raji cell lysates (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).
    生物信息
    免疫原品种 Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Thr4.
    表位 Phosphorylated Thr4
    克隆 1G7
    宿主 Rat
    特异性 This antibody recognizes RNAPII phosphorylated at Thr4.
    同种型 IgG2aκ
    品种反应性 YeastHumanMouse
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_000928
    基因符号
    • POLR2
    • POLR2A
    纯化方法 Protein G purified
    UniProt编号
    • P24928
    产品使用声明
    质量保证 Evaluated by Western Blot in untreated and lambda phosphatase treated HeLa cell lysates.

    Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-RNAPII (Thr4) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MABE348,Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

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