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品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, R, Ca WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT28 描述 Anti-Autotaxin Antibody Alternate Names - LysoPLD
- Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
- E-NPP 2
- Autotaxin
- Extracellular lysophospholipase D
背景信息 Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness. 产品信息 格式 Affinity Purified 控制 - Oligodendrocyte precursor cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.
Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region. 表位 Substrate binding region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Autotaxin at the substrate binding region. 品种反应性 BovineHumanMouseRatCanine Species Reactivity Note Demonstrated to react with Human.
Predicted to react with Mouse, Rat, Canine, and Bovine based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001035181
Entrez基因汇总 The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]. 基因符号 - PDNP2
- ENPP2
- ATX
纯化方法 Affinity Purified UniProt编号 - Q13822
UniProt汇总 FUNCTION: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
CATALYTIC ACTIVITY: 1-alkyl-sn-glycero-3-phosphoethanolamine + H2O = 1-alkyl-sn-glycerol 3-phosphate + ethanolamine.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
Binds 1 calcium ion per subunit (By similarity).
ENZYME REGULATION: Inhibited by lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P). Inhibited by EDTA and EGTA Probable.
SUBCELLULAR LOCATION: Secreted. Note: Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells.
TISSUE SPECIFICITY: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.
INDUCTION: Up-regulated in massively obese subjects with glucose intolerance, and during adipogenesis.
PTM: N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity (By similarity).
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
SEQUENCE SIMILARITIESl Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:
KM=0.5 mM for 16:0-LPC (at pH 8.5)
KM=5.5 mM for pNP-TMP (at pH 8.5)
KM=11.3 mM for pNppp (isoform 1)
KM=5.7 mM for pNppp (isoform 2)
KM=19.8 mM for pNppp (isoform 3)
Vmax=1.9 nmol/min/µg enzyme with pNppp as substrate (isoform 1)
Vmax=0.67 nmol/min/µg enzyme with pNppp as substrate (isoform 2)
Vmax=1.6 nmol/min/µg enzyme with pNppp as substrate (isoform 3)
pH dependence:
Optimum pH is 9.0 (isoform 1), 8.0 (isoform 3). Isoform 1 is less sensitive to pH. Isoform 1, isoform 2 and isoform 3 all retain some activity at pH 9.5.
Temperature dependence:
Isoform 1 and isoform 3 are active from 45 to 60 degrees Celsius.产品使用声明 质量保证 Evaluated by Western Blot in oligodendrocyte precursor cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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