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品种反应性 主要应用 宿主 格式 抗体类型 R, M IHC, ICC Rb AlexaFluor®488 Polyclonal Antibody 描述 产品目录编号 ABN78A4 描述 Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate | ABN78A4 Alternate Names - Neuronal Nuclei
- NEUronal Nuclei
背景信息 ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. 产品信息 格式 AlexaFluor®488 控制 - Adult mouse brain tissue
演示 Purified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 主要应用 - Immunohistochemistry
- Immunocytochemistry
应用说明 Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected NeuN in rat E18 primary cortex cells. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to mouse NeuN. 表位 N-terminus 宿主 Rabbit 特异性 This antibody recognizes NeuN at the N-terminus. 品种反应性 RatMouse Species Reactivity Note Demonstrated to react with Mouse and Rat. The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human. 抗体类型 Polyclonal Antibody 基因符号 - NeuN
纯化方法 Affinity purified 产品使用声明 质量保证 Evaluated by Immunohistochemistry in adult mouse brain tissue.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µL
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