merck millipore,默克密理博,48-611MAG,MILLIPLEX MAP Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit

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merck millipore,默克密理博,48-611MAG,MILLIPLEX MAP Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay

可用的分析物	品种反应性	主要应用	检测方法
TSC2 (Ser939), Akt (Ser473), GSK3α (Ser21), GSK3β (Ser9), IGF1R (Tyr1135/Tyr1136), IR (Tyr1162/Tyr1163), IRS1 (Ser312), mTOR (Ser2448), p70S6K (Thr412), PTEN (Ser380), RPS6 (Ser235/Ser236)	H	Mplex	Luminex xMAP

描述
产品目录编号	48-611MAG
商名	MILLIPLEX
描述	MILLIPLEX MAP Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay
概述	The Akt signaling pathway, one of the most often dysregulated signaling pathways in cancer, plays an important role in mediating a very broad range of cellular processes such as growth and development, cell cycle, energy homeostasis, and cell survival. Akt, a serine/threonine kinase that phosphorylates over 100 protein substrates, mediating cell survival and proliferation signals, is often itself hyperphosphorylated in various tumor types. Although the Akt gene itself is not known to be mutated in cancers, many of the upstream regulators of Akt, including IR, IRS, PI3K and PTEN, are oncogenes and tumor suppressors. Downstream of Akt, the mammalian Target Of Rapamycin (mTOR) complex is a key regulator of growth and metabolism. As nearly all of the players in the Akt/mTOR signaling pathway are coordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.

应用
应用	The analytes available for this multiplex kit are: Akt (Ser473), GSK3α (Ser21), GSK3β (Ser9), IGF1R (Tyr1135/Tyr1136), IR (Tyr1162/Tyr1163), IRS1 (Ser312), mTOR (Ser2448), p70S6K (Thr412), PTEN (Ser380), RPS6 (Ser235/Ser236), TSC2 (Ser939).
主要应用	Multiplexing
应用说明	• An overnight (4°C) incubation is recommended for best results. • This assay requires 25 µL diluted cell lysate per well. • This kit must be run using Assay Buffer 2 (provided). • The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).

生物信息
特异性	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
品种反应性	Human
可用的分析物	TSC2 (Ser939) Akt (Ser473) GSK3α (Ser21) GSK3β (Ser9) IGF1R (Tyr1135/Tyr1136) IR (Tyr1162/Tyr1163) IRS1 (Ser312) mTOR (Ser2448) p70S6K (Thr412) PTEN (Ser380) RPS6 (Ser235/Ser236)

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Recommended storage for kit components is 2 - 8°C.

包装信息
数量	96-well plate
包装	96-well plate

merck millipore,默克密理博,48-611MAG,MILLIPLEX MAP Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay

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