merck millipore,默克密理博,04-1075,Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1075,Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, rabbit monoclonal
产品名称：Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, rabbit monoclonal
产品型号：04-1075
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-GSK3 β (Ser9) using this Anti-phospho More>>

merck millipore,默克密理博,04-1075,Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1075,Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1075
描述	Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, rabbit monoclonal
Alternate Names	glycogen synthase kinase-3 beta GSK-3 beta GSK3beta isoform glycogen synthase kinase 3 beta
背景信息	GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.

产品信息
格式	Unpurified
控制	293T cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-GSK3 β (Ser9) using this Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, Rabbit validated for use in WB, IH(P) & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected GSK3 β in human ovarian carcinoma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot worked in IC.

生物信息
免疫原品种	Phospho-specific peptide corresponding to residues surrounding Ser9 of human GSK3 β.
表位	Phosphorylated Ser9
克隆	EPR2286Y
宿主	Rabbit
特异性	The antibody only detects GSK3 β phosphorylated on Ser9.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002084.2
Entrez基因汇总	Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).
基因符号	EC 2.7.11.26
修改	Phosphorylation
纯化方法	Unpurified
UniProt编号	P49841
UniProt汇总	FUNCTION: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Ref.5 CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate. ENZYME REGULATION: Inhibited when phosphorylated by AKT1. SUBUNIT STRUCTURE: Monomer By similarity. Interacts with CABYR, MUC1, NIN and PRUNE. TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. PTM: Phosphorylated by AKT1 and ILK1. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot in untreated and calyculin-treated 293T cell lysates. Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect GSK3 β in calyculin-treated 293T cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.