merck millipore,默克密理博,HTS217RTA,Ready-to-Assay™ GPR39 Orphan Receptor Frozen Cells

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	HEK293	GPR39

描述
产品目录编号	HTS217RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ GPR39 Orphan Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. GPR39 is a 7TM protein related to the G protein-coupled receptors for the gastrointestinal peptides ghrelin and motilin. Although GPR39 has been reported to be a receptor for the peptide obestatin, GPR39 has been more thoroughly characterized as a receptor for Zn2+, as well as Cu2+ and Ni2+ (Holst et al., 2007; Yasuda et al., 2007). Studies on GPR39-null mice implicate GPR39 in the control of insulin secretion, thereby indicating its potential as a target for treatment of diabetes (Holst et al., 2009; Tremblay et al., 2009). Millipore’s cloned human GPR39-expressing cell line is made in the HEK293 host, which supports high levels of recombinant GPR39 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GPR39.

产品信息
部件	Fifty (50) mL of Media Component. Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
检测方法	Fluorescent

应用
应用	Human GPR39 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.