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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE440 描述 Anti-RAG-1 Antibody Alternate Names - RING finger protein 74
- V(D)J recombination-activating protein 1
- RAG-1
背景信息 V(D)J recombination-activating protein 1 (RAG-1) is also called RING finger protein 74. RAG-1 is a component of the RAG complex, which mediates the DNA cleavage phase during V(D)J recombination. As part of the RAG complex, RAG1 mediates the DNA-binding and catalyzes the DNA cleavage activities by introducing a double-strand breaks between the recombination signal sequences (RSS) and the adjacent coding segment. RAG-1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3 as well as polyubiquitination of KPNA1. RAG-1 is expressed in maturing lymphoid cells. RAG-1 defects can cause disease states such as: combined cellular and humoral immune defects with granulomas (CHIDG), severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T-B-NK+ SCID), Omenn syndrome (OS), and alpha/beta T-cell lymphopenia with gamma/delta T-cell expansion severe cytomegalovirus infection and autoimmunity (T-CMVA). 产品信息 格式 Affinity Purified 演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blotting Analysis: 1 µg/mL from a representative lot detected RAG-1 immunopreciptated from 500 µg of HL-60 cell lysate. (Note: It is recommended to immunoprecipitate RAG-1 before performing WB).
Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected RAG-1 in Jurkat cells.
DyLight® is a registered trademark of Thermo Fisher Scientific.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to a region near the N-terminus of human RAG-1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000439
基因符号 - RNF74
- RAG1
纯化方法 Affinity purified UniProt编号 - P15918
产品使用声明 质量保证 Evaluated by Immunoprecipitation in HL-60 cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RAG-1 in 500 µg of HL-60 cell lysate. Immunopreciptated protein was then probed with 1 µg/mL of the same antibody in WB.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,397100,HNMPA-(AM)₃ - CAS 120944-03-8 - Calbiochem
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