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重要规格表
宿主 Rb 描述 概述 Recognizes the ~113 kDa PGC-1α protein in BAT cells. 产品目录编号 ST1204 品牌系列 Calbiochem®
应用数据 
Detection of mouse PGC-1α by immunoprecipitation followed by immunoblotting.
Samples:
Lane 1: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at room temperature, immunoprecipitated with 2 µg Anti-PGC-1α Rabbit pAb (Cat. No. ST1204).
Lane 2: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at 4°C for 5 hr, immunoprecipitated with 2 µg Anti-PGC-1α Rabbit pAb (Cat. No. ST1204).
Lane 3: Whole cell lysate from CHO cells overexpressing NT-PGC-1α positive control.
Lane 4: Whole cell lysate from COS cells overexpressing PGC-1α positive control.
Lane 5: Whole tissue extract (200 µg) from brown adipose tissue of a mouse held at 4°C for 5 hr, immunoprecipitated using Rabbit IgG negative control.
Lane 6: Whole tissue extract (10% input) from brown adipose tissue of a mouse held at room temperature (no IP).
Lane 7: Whole tissue extract (10% input) from brown adipose tissue of a mouse held at 4°C for 5 hr (no IP).
Antibody for immunoblotting: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.参考 参考 Chang, J.S., et al. 2010. J. Biol. Chem. 285, 18039.
Zhang, Y., et al. 2009. J. Biol. Chem. 284, 32813.
Lai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.产品信息 形式 Liquid 配方 In 50 mM PBS 正控制 Brown adipose tissue from mice exposed to cold 防腐剂 None 应用 主要应用 Immunoprecipitation
Not Western Blot
生物信息 免疫原 A recombinant protein consisting of amino acids 1-120 of mouse PGC-1α 免疫原品种 Mouse 宿主 Rabbit 同种型 IgG 品种 human, mouse, rat 储存和货运信息 货运代码 Dry Ice Only
毒性 Regulatory Review
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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下一件merck millipore产品：merck millipore,默克密理博,ABE440,Anti-RAG-1 Antibody
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