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merck millipore,默克密理博,525282,PhosphoDetect™ Phosphoserine Detection Kit
产品名称:PhosphoDetect™ Phosphoserine Detection Kit
产品型号:525282
This PhosphoDetect™ Phosphoserine Detection Kit is validated for use in Immunoblotting, Immunoprecipitation, ELISA.
merck millipore,默克密理博,525282,PhosphoDetect™ Phosphoserine Detection Kit
- 产品介绍
重要规格表
描述 概述 A set of four different monoclonal antibodies specific for serine phosphorylation sites. Recognition is dependent on phosphorylation and the surrounding amino acid motif. Recognizes serine phosphorylated proteins in Arabidopsis, bacteria, chicken, human, maize, mouse, rat, tobacco, Xenopus, yeast, and zebrafish. Phosphorylation patterns in cell extracts may differ when probed with different antibodies. 产品目录编号 525282 品牌系列 Calbiochem®
应用数据 

Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Whole cell lysate from A431 cells treated with pervanadate (all lates). Primary antibodies: PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (1C8) (Cat. No. 525281) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A3) (Cat. No. 525283) (1 µg/ml), and PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A9) (Cat. No. 525284) (1 µg/ml). Detection: chemiluminescence.
Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibodies: PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (1C8) (Cat. No. 525281) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A3) (Cat. No. 525283) (1 µg/ml), and PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A9) (Cat. No. 525284) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody.产品信息 形式 Lyophilized 配方 25 µg each antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl control phosphoproteins lyophilized 20 mM Na₂HPO₄, 0.1% SDS. 试剂盒包含 25 μg of each of the Phosphoserine Antibodies including clones 1C8, 4A3, 4A9, and 16B4, Rabbit Muscle Positive Control, and a data sheet. 正控制 Phosphoproteins purified from rabbit muscle (included) 防腐剂 ≤ 0.1% sodium azide (antibodies only) 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 phosphoserine-containing peptides or phosphoserine conjugated to KLH 宿主 Mouse 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Reconstitute each antibody with 1 ml H₂O (15 min, room temperature). Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C. Thaw aliquots at 37°C prior to use. Reconstitute the positive control with 200 µl H₂O. After complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate for 5 min at 90°C. Following incubation, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of all solutions. 补充信息 试剂盒包含 25 μg of each of the Phosphoserine Antibodies including clones 1C8, 4A3, 4A9, and 16B4, Rabbit Muscle Positive Control, and a data sheet.
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