merck millipore,默克密理愽
  • merck millipore,默克密理博,ABE419,Anti-Histone H3 Antibody, K27M mutant

  • merck millipore,默克密理博,ABE419,Anti-Histone H3 Antibody, K27M mutant

    产品名称：Anti-Histone H3 Antibody, K27M mutant
    产品型号：ABE419
    Anti-Histone H3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).

    merck millipore,默克密理博,ABE419,Anti-Histone H3 Antibody, K27M mutant

  • 产品介绍
  • merck millipore,默克密理博,ABE419,Anti-Histone H3 Antibody, K27M mutant

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    H, MChIP, WBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 ABE419
    描述 Anti-Histone H3 Antibody, K27M mutant
    Alternate Names
    • Histone H3.3
    • Histone H3.1
    背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
    产品信息
    格式 Affinity Purified
    控制
    • Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Anti-Histone H3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).
    主要应用
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    应用说明 Western Blotting Analysis: A representative lot detected histone H3 K27M mutant in diffuse intrinsic pontice Glioma tissue lysates expressing Histone H3 K27M mutant. (Lewis, P. W., et al. (2013). Science. 340(6134):857-861.

    Chromatin Immunoprecipitation: A representative lot co-precipitated chromatin fragments containing the promoter regions of ACTA and CCT8 genes from HEK293T transfectants expressing FLAG-HA-tagged histone H3.3 with K27M mutation (Peter W. Lewis and David Allis, Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY).
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3 with K27M mutation.
    表位 Histsone H3 sequence surrounding K27M mutation
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes Histone H3 with K27M mutation.
    品种反应性 HumanMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_002098
    基因符号
    • H3.3B
    • H3F3A
    • H3.3A
    • H3F3
    • PP781
    • H3F3B
    纯化方法 Immunogen affinity purified
    UniProt编号
    • P84243
    产品使用声明
    质量保证 Evaluated by Western Blotting in MEF (H3.3) and MEF (H3.3 K27M-HA-Flag) transfected cell lysate.

    Western Blotting Analysis: 0.2 µg/mL of this antibody detected FLAG-HA-tagged K27M mutant, but not wildtype, histone H3 in 3x10E5 cell equivalent of lysate from MEF transfectants.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,ABE419,Anti-Histone H3 Antibody, K27M mutant

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