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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-324 品牌系列 Upstate
商名 - Upstate
描述 Anti-PP2A Antibody, C subunit 产品信息 格式 Affinity Purified 演示 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30% 应用 应用 This Anti-PP2A Antibody, C subunit is validated for use in WB for the detection of PP2A. 主要应用 - Western Blotting
生物信息 免疫原品种 peptide corresponding to amino acids 290-304 (DPAPRRGEPHVTRRT) of human PP2A 宿主 Rabbit 特异性 PP2A catalytic subunit 同种型 IgG 品种反应性 RatBovineHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002715, NM_004156
Entrez基因汇总 This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. 基因符号 - PP2Ac
- PPP2CA
- PP2CA
- PP2A-alpha
- RP-C
纯化方法 Immunoaffinity chromatography UniProt编号 - P67775
UniProt汇总 FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 309 amino acids; 35594 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from mouse brain, heart, testes and spleen homogenates 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 100 µL
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