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品种反应性 主要应用 宿主 格式 抗体类型 H IHC, ELISA, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB19012 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Laminin Antibody 产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide. 应用 应用 Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Immunoblot: 1:500-1:2000
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE. 宿主 Rabbit 特异性 This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005559.2
基因符号 - LAMA
- LAMA1
UniProt编号 - P25391
UniProt汇总 FUNCTION: SwissProt: P25391 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
SIZE: 3075 amino acids; 337158 Da
SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-1 is a subunit of laminin-1 (EHS laminin) and laminin-3 (S-laminin).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
DOMAIN: SwissProt: P25391 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular.
SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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