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  • merck millipore,默克密理博,ABE153,Anti-phospho Histone H3.3 Antibody (Thr32)

  • merck millipore,默克密理博,ABE153,Anti-phospho Histone H3.3 Antibody (Thr32)

    产品名称：Anti-phospho Histone H3.3 Antibody (Thr32)
    产品型号：ABE153
    Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.3 Antibody (Thr32) validated for use in western blotting, IP & Dot Blot.

    merck millipore,默克密理博,ABE153,Anti-phospho Histone H3.3 Antibody (Thr32)

  • 产品介绍
  • merck millipore,默克密理博,ABE153,Anti-phospho Histone H3.3 Antibody (Thr32)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 ABE153
    描述 Anti-phospho Histone H3.3 Antibody (Thr32)
    Alternate Names
    • Histone H3.3
    背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
    产品信息
    格式 Affinity Purified
    控制
    • Untreated and Colcemid-treated HeLa cell lysates, and in Histone H3 recombinant protein
    演示 Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.3 Antibody (Thr32) validated for use in western blotting, IP & Dot Blot.
    主要应用
    • Western Blotting
    应用说明 Dot Blot (Specificity) Analysis: 0.2 µg/mL of this antibody detected phospho Histone H3.3 (Thr32), but not non-phospho Histone H3 (Thr32), or other peptides corresponding to modified histones.

    Immunoprecipitation Analysis: A representative lot detected phospho Histone H3.3 (Thr32) in IP.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3.3 phosphorylated at Thr32.
    表位 Near N-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes a region near the N-terminus of Histone H3.3 phosphorylated at Thr32. This antibody demonstrates specificity for phospho Histone H3.3 (Thr32), but does not cross react with non-phospho Histone H3.3 (Thr32) or any other Histone proteins.
    品种反应性 Human
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_002098
    基因符号
    • H3.3B
    • H3F3A
    • H3.3A
    • H3F3
    • H3F3B
    纯化方法 Affinity purified
    UniProt编号
    • P84243
    产品使用声明
    质量保证 Evaluated by Western Blotting in untreated and Colcemid-treated HeLa cell lysates, and in Histone H3 recombinant protein.

    Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3.3 (Thr32) in 10 µg of Colcemid-treated HeLa cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,ABE153,Anti-phospho Histone H3.3 Antibody (Thr32)

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