merck millipore,默克密理博,07-549-S,Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size

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merck millipore,默克密理博,07-549-S,Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size
产品名称：Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size
产品型号：07-549-S
Use Anti-trimethyl-Histone H3 (Lys36), Trial Size Antibody (Rabbit Polyclonal Antibody) validated in DB, WB to detect trimethyl-Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).

merck millipore,默克密理博,07-549-S,Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size

产品介绍

merck millipore,默克密理博,07-549-S,Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	07-549-S
品牌系列	Upstate
商名	Upstate
描述	Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size
Alternate Names	H3 histone, family 3A H3K36me3 Histone H3 (tri methyl K36)
背景信息	Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation.

产品信息
格式	Serum
控制	Acid extracted proteins from HeLa cells.
演示	Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

应用
应用	Use Anti-trimethyl-Histone H3 (Lys36), Trial Size Antibody (Rabbit Polyclonal Antibody) validated in DB, WB to detect trimethyl-Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated, synthetic peptide containing the sequence…GVme3KKP…in which me3K corresponds to trimethyl lysine 36 of human histone H3.
表位	Lys36
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes trimethyl-histone H3 (Lys36), Mr ~17 kDa. A non-specific protein was also detected, Mr ~78 kDa.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human. Broad species cross-reactivity is expected.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_003484
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
基因符号	H3F3B H3F3A MGC87783 H3.3A MGC87782 H3F3 H3.3B
修改	Methylation
纯化方法	Unpurified
UniProt编号	Q16695
UniProt汇总	FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.

产品使用声明
质量保证	Routinely evaluated by western blot chicken core histones Western Blot Analysis: A 1:500-1:2000 dilution of this lot detected trimethyl-Histone H3 (Lys36) chicken core histones (Catalog # 13-107). The antibody did not detect unmodified recombinant Histone H3 (Catalog # 14-411)
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.