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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Fe, B, Ca, H, R, Sh IH(P), IF Rb Serum Polyclonal Antibody 描述 产品目录编号 AB960 Replaces CBL78 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Prolactin Antibody 产品信息 格式 Serum 演示 Liquid antiserum. Contains 0.1% sodium azide. 应用 应用 Detect Prolactin using this Anti-Prolactin Antibody validated for use in IF, IH(P). 主要应用 - Immunohistochemistry (Paraffin)
- Immunofluorescence
应用说明 TISSUE EVALUATION:
Formalin-fixed and paraffin-embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including human, dog, rat, cat, ox and sheep. Cryostat sections (10 microns thick) of the above tissues can also be used in the staining procedures.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:1,000 - 1:2,000 (in PBS) is allowed to react with the tissue at 37°C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with sheep anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above.
4. The final step in the staining involves treating the section with a mixture of 3,3'-diaminobenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C.
3. After washing off the excess reagents with PBS, the section is treated with fluorescein labeled goat anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 minutes at 37° C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope.
COMMENTS:
The antiserum dilutions described have been established as giving a positive staining on the lactotrophs when using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr or 4° C.生物信息 免疫原品种 ANTIGEN:
The antigen used in the production of the antiserum has been purified from sheep pituitary glands.宿主 Rabbit 特异性 The antiserum provided has been evaluated for its ability to give a specific and positive immunostaining of prolactin-containing cells using both the immunoperoxidase and immunofluorescence procedures. 品种反应性 FelineBovineCanineHumanRatSheep 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000948.3
基因符号 - prolactin
- PRL
UniProt编号 - P01236
UniProt汇总 FUNCTION: SwissProt: P01236 # Prolactin acts primarily on the mammary gland by promoting lactation.
SIZE: 227 amino acids; 25876 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01236 ## Belongs to the somatotropin/prolactin family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 500 µL
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