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检测方法 Colorimetric 描述 概述 Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required. 产品目录编号 QIA127 品牌系列 Calbiochem®
参考 参考 Ishiyama, M., et al. 1995. In Vitro Toxicology 8, 187.
Liu, S.O., et al 1995. Nat. Med. 1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull. 41, 1118.产品信息 检测方法 Colorimetric 形式 500 Tests 格式 96-well plate 试剂盒包含 WST-1, 1-Methoxy PMS, and a user protocol. 生物信息 化验范围 1000 - 50,000 Cells 化验时间 0.5 - 4 h 样品类型 Cultured cells 理化信息 灵敏度 1000 cells (adherent); 2500 cells (nonadherent) 产品使用声明 预定用途 The Calbiochem® Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
存储条件 Upon arrival store the entire contents of the kit at -20°C. 避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 WST-1, 1-Methoxy PMS, and a user protocol.
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