merck millipore,默克密理愽
  • merck millipore,默克密理博,AB1587P,Anti-Adenosine A1 Receptor Antibody

  • merck millipore,默克密理博,AB1587P,Anti-Adenosine A1 Receptor Antibody

    产品名称：Anti-Adenosine A1 Receptor Antibody
    产品型号：AB1587P
    Anti-Adenosine A1 Receptor Antibody detects level of Adenosine A1 Receptor & has been published & validated for use in ELISA, IC, IH & WB.

    merck millipore,默克密理博,AB1587P,Anti-Adenosine A1 Receptor Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB1587P,Anti-Adenosine A1 Receptor Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Sh, Gp, H, Po, RWB, ELISA, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB1587P
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Adenosine A1 Receptor Antibody
    产品信息
    格式 Affinity Purified
    控制
    • Brain tissue
    演示 Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
    应用
    应用 Anti-Adenosine A1 Receptor Antibody detects level of Adenosine A1 Receptor & has been published & validated for use in ELISA, IC, IH & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 10-50 μg/mL on frozen tissue sections. Light fixation is recommended. 2% 0.2% picric acid {Cooke, HJ (1999) AJP Gastrointest Liver Physiol 276:4516-4626}

    Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

    Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.

    ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Amino acid sequence corresponding to the 3rd extracellular domain (amino acids 163-176, GEPVIKCEFEKVIS) of the rat A1R gene.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Adenosine A1 receptor (A1R). Recognizes the 79 kDa and 39 kDa bands of in cortical membranes from rat, porcine and lamb. The 79 kDa band can be converted to 39 kDa band by agonist or antagonist treatment and could represent a A1R dimers.
    品种反应性 SheepGuinea PigHumanPigRat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_001048230.1
    • NM_000674.2
    Entrez基因汇总 The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene.
    基因符号
    • RDC7
    • ADORA1
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P30542
    UniProt汇总 FUNCTION: SwissProt: P30542 # Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
    SIZE: 326 amino acids; 36512 Da
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    SIMILARITY: SwissProt: P30542 ## Belongs to the G-protein coupled receptor 1 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,AB1587P,Anti-Adenosine A1 Receptor Antibody

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