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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9082 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Ryanodine Receptor 3 Antibody 产品信息 格式 Serum 控制 - Western blot = adult mouse brain (adult mouse skeletal muscle will be negative).
IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).
演示 Rabbit serum. Liquid. 应用 应用 This Anti-Ryanodine Receptor 3 Antibody is validated for use in IC, IH, WB for the detection of Ryanodine Receptor 3. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 3 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the variant TM region of human Ryanodine Receptor 3. 宿主 Rabbit 特异性 Ryanodine Receptor 3. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001036.3
Entrez基因汇总 Ryanodine receptors, such as RYR3, are intracellular calcium ion release channels responsible for the release of Ca(2+) from intracellular stores following transduction of many different extracellular stimuli.[supplied by OMIM] 基因符号 - RyR3
- RYR3
- RYR-3
- HBRR
UniProt编号 - Q15413
UniProt汇总 FUNCTION: SwissProt: Q15413 # Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules (By similarity).
SIZE: 4870 amino acids; 551932 Da
SUBUNIT: Homotetramer (Potential).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
TISSUE SPECIFICITY: Brain, skeletal muscle, placenta and possibly liver and kidney. In brain, highest levels are found in the cerebellum, hippocampus, caudate nucleus and amygdala, with lower levels in the corpus callosum, substantia nigra and thalamus.
SIMILARITY: SwissProt: Q15413 ## Belongs to the ryanodine receptor family. & Contains 3 B30.2/SPRY domains. & Contains 1 EF-hand domain. & Contains 5 MIR domains.
MISCELLANEOUS: The calcium release channel is modulated by calcium ions, magnesium ions, ATP and calmodulin. & The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the SR and the T-tubule. It is possible that the foot structure interacts with the cytoplasmic region of the dihydropyridine receptor. & Ryanodine is an alkaloid that binds to the Ca- release channel in junctional SR and modulates its activity.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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