merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5898,Anti-Protein Gene Product 9.5 Antibody

  • merck millipore,默克密理博,AB5898,Anti-Protein Gene Product 9.5 Antibody

    产品名称：Anti-Protein Gene Product 9.5 Antibody
    产品型号：AB5898
    Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.

    merck millipore,默克密理博,AB5898,Anti-Protein Gene Product 9.5 Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5898,Anti-Protein Gene Product 9.5 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MIHCGpSerumPolyclonal Antibody
    描述
    产品目录编号 AB5898
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Protein Gene Product 9.5 Antibody
    Alternate Names
    • UCH-L1
    • PGP 9.5
    产品信息
    格式 Serum
    控制
    • Brain tissue
    演示 Liquid. Contains 0.05% sodium azide.
    应用
    应用 Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.
    主要应用
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 1:500.

    Optimal working dilutions must be determined by end user.

    blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
    生物信息
    免疫原品种 A 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Guinea Pig
    特异性 Recognizes Protein Gene Product 9.5 (PGP9.5).
    品种反应性 RatHumanMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_004181.3
    Entrez基因汇总 UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]
    基因符号
    • EC 6.-.-.-
    • UCHL1
    • PARK5
    • UCH-L1
    • Uch-L1
    • PGP9.5
    • EC 3.4.19.12
    纯化方法 Unpurified
    UniProt编号
    • P09936
    UniProt汇总 FUNCTION: SwissProt: P09936 # Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
    SIZE: 223 amino acids; 24824 Da
    SUBUNIT: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.
    SUBCELLULAR LOCATION: Cytoplasm.
    TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary.
    PTM: O-glycosylated (By similarity).
    DISEASE: SwissProt: P09936 # Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.
    SIMILARITY: SwissProt: P09936 ## Belongs to the peptidase C12 family.
    MISCELLANEOUS: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5898,Anti-Protein Gene Product 9.5 Antibody

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