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merck millipore,默克密理博,AB5830,Anti-8-Hydroxydeoxyguanosine Antibody
产品名称：Anti-8-Hydroxydeoxyguanosine Antibody
产品型号：AB5830
This Anti-8-Hydroxydeoxyguanosine Antibody is validated for use in ELISA, IH(P) for the detection of 8-Hydroxydeoxyguanosine.
merck millipore,默克密理博,AB5830,Anti-8-Hydroxydeoxyguanosine Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ELISA Gt Serum Polyclonal Antibody 描述 产品目录编号 AB5830 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-8-Hydroxydeoxyguanosine Antibody Alternate Names - 8-hydroxy-2'-deoxyguanosine
- 8-OHdG
背景信息 8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.
8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.
CHEMICON's new goat antiserum to 8-hydroxyguanosine is now available. This antiserum has been shown by ELISA to be completely cross-reactive with 8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring nucleotides. This antibody should be a valuable tool for scientists working to understand the role of free radical damage in a number of human disease states.产品信息 格式 Serum 控制 - Skin, staining is limited to the nucleus.
演示 Goat Serum. Liquid containing 0.01% Thimerosal. 应用 应用 This Anti-8-Hydroxydeoxyguanosine Antibody is validated for use in ELISA, IH(P) for the detection of 8-Hydroxydeoxyguanosine. 主要应用 - ELISA
应用说明 Immunohistochemistry: >1:200 on paraffin embedded, formalin fixed human brain. It is suggested that the tissues are pretreated with 10 μg/mL of proteinase K for 40 minutes at 37°C prior to staining.
Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% NGS in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Goat 特异性 Reacts with 8-Hydroxydeoxyguanosine (8-OHdG) and 8-Hydroxyguanosine (8-OHG). By a competitive ELISA the antibody was 100% inhibited by 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Partial cross-reactivity was observed with 8-mercaptoguanosine (80%) while 8-bromoguanosine showed some cross-reactivity (5%). 2-Deoxyadenosine, 7-methlyguanosine, guanosine monophosphate and guanosine showed no reactivity. 品种反应性 Human 抗体类型 Polyclonal Antibody 纯化方法 Unpurified 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µL
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