merck millipore,默克密理博,04-1114,Anti-TNF alpha Antibody, clone EP1085Y, rabbit monoclonal

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merck millipore,默克密理博,04-1114,Anti-TNF alpha Antibody, clone EP1085Y, rabbit monoclonal
产品名称：Anti-TNF alpha Antibody, clone EP1085Y, rabbit monoclonal
产品型号：04-1114
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect TNF alpha using this Anti-TNF alpha Antibody, More>>

merck millipore,默克密理博,04-1114,Anti-TNF alpha Antibody, clone EP1085Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1114,Anti-TNF alpha Antibody, clone EP1085Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1114
描述	Anti-TNF alpha Antibody, clone EP1085Y, rabbit monoclonal
Alternate Names	tumor necrosis factor alpha
背景信息	Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. GF023 is a recombinant polypeptide containing the mature form of human TNF-a with a polyhistidine tag at its amino terminal.

产品信息
格式	Unpurified
控制	Recombinant protein
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect TNF alpha using this Anti-TNF alpha Antibody, clone EP1085Y, Rabbit validated for use in WB.
主要应用	Western Blotting

生物信息
免疫原品种	Synthetic peptide corresponding to the extracellular portion of human TNF-α.
克隆	EP1085Y
宿主	Rabbit
特异性	This antibody recoganizes TNF alpha.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_000585.2
Entrez基因汇总	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
基因符号	TNFA TNF-a TNF-alpha
纯化方法	Unpurified
UniProt编号	P01375
UniProt汇总	FUNCTION: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. SUBUNIT STRUCTURE: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted. PTM: The soluble form derives from the membrane form by proteolytic processing. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. INVOLVEMENT IN DISEASE: Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SEQUENCE SIMILARITIES: Belongs to the tumor necrosis factor family. SEQUENCE CAUTION: The sequence AAF71992.1 differs from that shown. Reason: Frameshift at positions 91 and 157. The sequence CAA75070.1 differs from that shown. Reason: Erroneous gene model prediction.

产品使用声明
质量保证	Evaluated by Western Blotting on recombinant protein. Western Blotting Analysis: 1:500 dilution of this antibody was used to detect TNF alpha.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.