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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, ELISA Ch Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5745 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Opsin Antibody, Red/Green 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 Detect Opsin using this Anti-Opsin Antibody, Red/Green validated for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Immunoblotting: 1:500:1:2,000 on mouse brain extract. To minimize background staining it is suggested that you include a higher content of detergent (Tween-20) in your dilution and washing solutions.
Immunohistochemistry: not tested. It is recommended that you try the antibody at 1:50-1:1,000.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptides from mouse green opsin and zebra finch red opsin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Chicken 特异性 Opsin, red and green. The antibody reacts with a protein of ~40 kDa. 品种反应性 RatMouse Species Reactivity Note Rat and mouse.
Other species have not been tested.
It is expected that the antibody will also react with zebra finch.抗体类型 Polyclonal Antibody Entrez基因编号 - NM_020061.3
Entrez基因汇总 This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called red cone photopigment or long-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. This gene and the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of partial, protanopic colorblindness. 基因符号 - RCP
- OPN1LW
- CBP
- CBBM
UniProt编号 - P04000
UniProt汇总 FUNCTION: SwissProt: P04000 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
SIZE: 364 amino acids; 40572 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE:SwissProt: P04000 # Defects in OPN1LW are the cause of partial colorblindness protan series (CBP) [MIM:303900]; also called protanopia.SIMILARITY:SwissProt: P04000 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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