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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Fe, B, H, M, Po, R WB, ICC, IHC Ch Purified Polyclonal Antibody 描述 产品目录编号 AB5539 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament H Antibody Alternate Names - NF-H
产品信息 格式 Purified 控制 - Positive control: brain.
Negative control: kidney, liver.
演示 Purified chicken IgY in PBS containing 5mM sodium azide. 应用 应用 Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Purified bovine NF-H. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Chicken 特异性 Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons. 品种反应性 FelineBovineHumanMousePigRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_021076.2
基因符号 - KIAA0845
- NEFH
- NF-H
- NFH
纯化方法 Ammonium sulfate precipitation UniProt编号 - P12036
UniProt汇总 FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 50 µL
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