merck millipore,默克密理博,MAB3751,Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1

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merck millipore,默克密理博,MAB3751,Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1
产品名称：Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1
产品型号：MAB3751
Detect LEF-1 using this Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1 validated for use in IF & WB.

merck millipore,默克密理博,MAB3751,Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1

产品介绍

merck millipore,默克密理博,MAB3751,Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB3751
Replaces	04-1130
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1
Alternate Names	Lymphoid Enhancer Factor 1
背景信息	Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kDa on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB1 recognizes the b-catenin binding domain of LEF-1.

产品信息
格式	Purified
控制	Jurkat whole cell lysate, CCRF-HSB-2 (T lymphoblast) cell lysate, HuT 78 (cutaneous T lymphocyte) whole cell lysate
演示	Purified ascites. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide.

应用
应用	Detect LEF-1 using this Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1 validated for use in IF & WB.
主要应用	Western Blotting
应用说明	Western blot (1-5 μg/ml) Immunofluoresnce Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	The immunogen for the anti-LEF-1 antibody was a full length protein. Does not cross-react with TCF-1 or TCF-4.
表位	beta catenin binding domain
克隆	REMB1
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes the b-catenin binding domain of LEF-1(Lymphoid Enhancer Factor-1).
同种型	IgG1
品种反应性	MouseHuman
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_016269.2
Entrez基因汇总	Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
基因符号	DKFZp586H0919 LEF1 LEF-1 TCF1-alpha TCF1ALPHA
UniProt编号	Q9UJU2
UniProt汇总	FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. SIZE: 399 amino acids; 44201 Da SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC. SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity). TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

包装信息
数量	250 µg

merck millipore,默克密理博,MAB3751,Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1

上一件merck millipore产品：merck millipore,默克密理博,07-2138,Anti-TDRD1 Antibody
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