merck millipore,默克密理博,AB5983,Anti-Nkx-3.1 Antibody

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merck millipore,默克密理博,AB5983,Anti-Nkx-3.1 Antibody
产品名称：Anti-Nkx-3.1 Antibody
产品型号：AB5983
Anti-Nkx-3.1 Antibody is an antibody against Nkx-3.1 for use in WB, IC, IH.

merck millipore,默克密理博,AB5983,Anti-Nkx-3.1 Antibody

产品介绍

merck millipore,默克密理博,AB5983,Anti-Nkx-3.1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	IHC, WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5983
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Nkx-3.1 Antibody

产品信息
格式	Affinity Purified
演示	Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Anti-Nkx-3.1 Antibody is an antibody against Nkx-3.1 for use in WB, IC, IH.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide, amino acids 70-83 of Nkx3.1.
宿主	Rabbit
特异性	Recognizes Nkx3.1. The molecular weight of the protein is 21,623 Daltons (v2 21,097, v3 19,077, v4 18,449).
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_006167.2
Entrez基因汇总	The homeodomain-containing transcription factor NKX3A is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3A protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.[supplied by OMIM]
基因符号	NKX3 NKX3-1 NKX3A NKX3.1 BAPX2
UniProt编号	Q99801
UniProt汇总	FUNCTION: SwissProt: Q99801 # Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Could play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate. SIZE: 234 amino acids; 26350 Da SUBUNIT: Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Highly expressed in the prostate and, at a lower level, in the testis. DISEASE:SwissProt: Q99801 # NKX3-1 has been thought to be one of the target gene of the 8p21 loss of heterozygosity, common in prostate cancer, but neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer. SIMILARITY: SwissProt: Q99801 ## Belongs to the NK-3 homeobox family. & Contains 1 homeobox DNA-binding domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

储存和货运信息

存储条件

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.