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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Zebrafish, H, M, R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3737 Replaces MABE554 描述 Anti-RBX1 Antibody Alternate Names - ring-box 1
- Protein ZYP
- RING box protein 1
- RING finger protein
- RING finger protein 75
- Regulator of cullins
- ZYP protein
背景信息 RING-box protein 1 RBX1 is an evolutionarily conserved protein that interacts with cullins. It plays a unique role in the ubiquitination reaction by forming a heterodimer with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.
RBX1 is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, RBX1 seems to recruit the E2 ubiquitination enzyme CDC34 to the complex and brings it into close proximity to the substrate and probably stimulates CDC34 autoubiquitination. It promotes the neddylation of CUL1 and probably CUL2. RBX1 may be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair.
产品信息 格式 Purified 控制 - Human testis lysate
演示 Purified in PBS containing 0.09% NaN3 应用 应用 Detect RBX1 using this Anti-RBX1 Antibody validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to amino acids 94-108 within the C-terminus of human RBX1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB3737 recognizes human RBX1. 品种反应性 ZebrafishHumanMouseRat Species Reactivity Note Human. Predicted to cross react with mouse, rat, and zebrafish, based on sequence homology Reactivity with other species has not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_014248
Entrez基因汇总 This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq] 基因符号 - Rbx1
- BA554C12.1
- MGC13357
- MGC1481
- OTTHUMP00000028983
- RNF75
- ROC1
纯化方法 Peptide affinity purified UniProt编号 - P62877
UniProt汇总 FUNCTION: Component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. Promotes the neddylation of CUL1 and probably CUL2. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of CBC(VHL) complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 or CUL5 and VHL. Part of multisubunit E3 ubiquitin ligase complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 and MED8; elongin BC complex (TCEB1 and TCEB2), CUL5 and MUF1. Part of multisubunit complexes with elongin BC complex (TCEB1 and TCEB2), elongin A/TCEB3 or SOCS1 or WSB1 and CUL5. Interacts directly with CUL1 and probably also with CUL2, CUL3, CUL4A, CUL4B, CUL5 and CUL7. Probably interacts with CDC34. Interacts with COPS6. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Part of an E3 ligase complex composed of RBX1, DDB1, DDB2 and CUL4A or CUL4B.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed.
DOMAIN: The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.产品使用声明 质量保证 Routinely evaluated by Western blot on human testis lysate using 0.5 g/mL of Anti-RBX1 at 0.5 mg/mL. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 1 year from date of receipt. 包装信息 数量 100 µg
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