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merck millipore,默克密理博,HTS129M,ChemiScreen™ D5 Dopamine Receptor Membrane Preparation
产品名称：ChemiScreen™ D5 Dopamine Receptor Membrane Preparation
产品型号：HTS129M
Human D5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS129M,ChemiScreen™ D5 Dopamine Receptor Membrane Preparation
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 D5 描述 产品目录编号 HTS129M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ D5 Dopamine Receptor Membrane Preparation 概述 Full-length human DRD5 cDNA encoding D5 背景信息 Dopamine is a catecholamine neurotransmitter that functions in the CNS to control locomotor, cognitive, emotional and neurendocrine processes, and in the periphery to modulate cardiovascular, renal and gastrointestinal processes. The biological activities of dopamine are mediated by a family of five GPCRs. The D1 and D5 subtypes couple to Gs to increase intracellular cAMP, whereas the D2, D3 and D4 subtypes couple to Gi to reduce cAMP (Missale et al., 1998). The hypertensive phenotype of mice with a targeted deletion of D5 indicates that D5 regulates central control of sympathetic vascular tone (Hollon et al., 2002). In addition, D5 modulates locomotion and corticostriatal long-term depression (Centonze et al., 2003). Chemicon's cloned human D5-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant D5 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between D5 and its ligands. The membrane preparations exhibit a Kd of 2.25 nM for [3H]-SCH 23390. With 0.5 nM [3H]-SCH 23390, 10 µg/well D5 Membrane Prep yields greater than 3-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to 1 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80%deg;C. 应用 应用 Human D5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_000798.3
Entrez基因汇总 This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. 基因符号 - MGC10601
- DRD5
- DRD1B
- DBDR
- DRD1L2
- D
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 D5 靶子系列 Dopamine UniProt编号 - P21918
UniProt汇总 FUNCTION: SwissProt: P21918 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 477 amino acids; 52951 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Neuron-specific, localized primarily within limbic regions of the brain.
DISEASE: SwissProt: P21918 # Defects in DRD5 may be a cause of schizophrenia, but no proof has yet been found. & Defects in DRD5 are a cause of blepharospasm [MIM:606798]. It is a primary focal dystonia affecting the orbicularis oculi muscles, usually beginning in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. In severe cases, this can lead to functional blindness.
SIMILARITY: SwissProt: P21918 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H]-SCH 23390. (Perkin Elmer#:NET-930 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H labeled SCH 23390 at 0.5 nM产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of D5 membrane prep:
10 µg/well Signal:Background 4.06 Specific Binding (cpm) 1952
SPECIFICATIONS: 1 unit = 10 µg
Bmax for [3H] SCH 23390 binding: 11.42 pmol/mg protein;
Kd for [3H] SCH 23390 binding: ~ 2.25 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,14-659,LIM Kinase 1 Protein, unactive, 50 µg
下一件merck millipore产品：merck millipore,默克密理博,69069,Cleavage Control Protein
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