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品种反应性 主要应用 宿主 格式 抗体类型 B, H, Ca IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2969 描述 Anti-Gelsolin Antibody Alternate Names - Brevin
- gelsolin
- gelsolin (amyloidosis, Finnish type)
- brevin
- Actin-depolymerizing factor
背景信息 Gelsolin is a calcium-dependent actin capping and severing protein belonging to the villin/gelsolin family and serves to regulate several cellular functions: remodeling actin cytoskeleton, motility, morphology, secretion, and apoptosis. It is able to bind actin filaments and monomers at the plus end, effectively inhibiting the exchange of monomers. It is also able to sever preformed filaments and increase nucleation. When cleaved by caspase-3, the N-terminal end of gelsolin is active and pro-apoptotic, promoting the depolarization of actin and leading to cell death. However, studies have shown overepxression of gelsolin effectively inhibits caspase-3 and can therefore have a role in cell survival. Expression has been observed in fibroblasts, nonmuscle cells, phagocytic cells, platelets, skeletal and smooth muscle cells. Mutations in gelsolin expression are causal to amyloidosis type 5 (AMYL5). High levels of expression have been linked to human heart failure progression. 产品信息 格式 Affinity Purified 控制 - MDCK cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Gelsolin Antibody detects level of Gelsolin & has been published & validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blot (Snap i.d.®) Analysis: 0.5 µg/mL from a previous lot detected Gelsolin in 10 µg of MDCK cell lysate.
Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected Gesolin in human colon adenocarcinoma tissue.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to a region within the actin-binding domain of human Gelsolin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 BovineHumanCanine Species Reactivity Note Demonstrated to react with human and canine. Predicted to react with bovine based on 100% sequence homology. Other homologies: Mouse and rat (95% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000168
Entrez基因汇总 The protein encoded by this gene binds to the 'plus' ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - AGEL
- ADF
- GSN
纯化方法 Affinity purified UniProt编号 - P06396
UniProt汇总 FUNCTION: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed.
SUBUNIT STRUCTURE: Binds to actin and to fibronectin.
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm › cytoskeleton.
Isoform 1: Secreted.
TISSUE SPECIFICTY: Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
PTM: Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
INVOLVEMENT IN DISEASE: Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
SEQUENCE SIMILARITIES: Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.产品使用声明 质量保证 Evaluated by Western Blot in MDCK cell lysate.
Western Blot Analysis: 0.5 µg/mL of the antibody detected Gelsolin in 10 µg of MDCK cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 50 µg
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