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重要规格表
主要应用 可用的分析物 检测方法 EA Tyrosine Kinase Colorimetric 描述 产品目录编号 17-315 品牌系列 Upstate
商名 - Upstate
描述 Tyrosine Kinase Assay Kit, Colorimetric Detection 概述 This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the well of a streptavidin-coated 96-well plate. The second stage involves the detection of phosphorylated substrate by Direct Enzyme Linked Immunosorbent Assay (ELISA) using a monoclonal anti-phosphotyrosine-HRP (Horse Radish Peroxidase) antibody conjugate. Tetramethylbenzidine (TMB) is provided as the HRP substrate. 产品信息 部件 - Sodium Orthovanadate, 50mM
- Streptavidin Coated Microwell Plate (Cat.# 20-183)
- Anti-pTyr, recombinant 4G10™, HRP
- ELISA wash and blocking buffers
- Poly (Glu4-Phospho-Tyr), biotinylated
- Poly (Glu4-Tyr), biotinylated
- Tyrosine Kinase Reaction Buffer, 5X
检测方法 Colorimetric 应用 主要应用 - Enzyme Assay
生物信息 可用的分析物 - Tyrosine Kinase
产品使用声明 质量保证 Routinely evaluated by a reference peptide titration. Increasing amounts of Poly (Glu4-phospho-Tyr) Peptide, biotinylated were bound to streptavidin-coated wells. Total peptide mass/well was adjusted by the addition of Poly (Glu4-Tyr) Peptide, biotinylated to a well. Bound phospho-tyrosine containing peptide was detected with Anti-Phosphotyrosine, recombinant 4G10, HRP conjugate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 1 kit
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下一件merck millipore产品：merck millipore,默克密理博,AB2969,Anti-Gelsolin Antibody
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