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品种反应性 主要应用 宿主 格式 抗体类型 R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB9298 Replaces AB5427 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MARCKS Antibody, Complete sequence 产品信息 格式 Purified 控制 - POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
演示 Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide. 应用 应用 Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB. 主要应用 - Western Blotting
应用说明 Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Full length recombinant rat MARCKS. 表位 Complete sequence 宿主 Rabbit 特异性 Recognizes MARCKS. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002356.5
Entrez基因汇总 The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. 基因符号 - FLJ90045
- MARCKS
- MACS
- 80K-L
- FLJ14368
- PRKCSL
- phosphomyristin
- MRACKS
- PKCSL
UniProt编号 - P29966
UniProt汇总 FUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SIZE: 332 amino acids; 31555 Da
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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