merck millipore,默克密理博,2060,ReBlot Western Blot Recycling Kit

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merck millipore,默克密理博,2060,ReBlot Western Blot Recycling Kit
产品名称：ReBlot Western Blot Recycling Kit
产品型号：2060

merck millipore,默克密理博,2060,ReBlot Western Blot Recycling Kit

产品介绍

merck millipore,默克密理博,2060,ReBlot Western Blot Recycling Kit

重要规格表

主要应用
WB

描述
产品目录编号	2060
品牌系列	Chemicon®
商名	Chemicon ReBlot
描述	ReBlot Western Blot Recycling Kit
概述	Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots. Stripping and re-probing of Western blots offers several advantages: 1) Conservation of samples that are expensive or available only in limited quantities, 2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies, 3) Re-analysis of anomalous results and confirmation with the same or a different antibody, 4) Correcting errors in incubation with the wrong antibody, 5) Cost savings in reagents and time by reusing the same blot. While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting. The MILLIPORE Re-Blot Western Blot Recycling Kit contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins. Advantages of the Re-Blot Western Blot Recycling Kit include: ·No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution. ·Antibody stripping is done at room temperature. No heating of blots is required. ·Blots can be stripped of antibodies in approximately 10 minutes at room temperature. ·Reblocking of blots may be avoided in most instances. ·*Blots may be reused in less than 15 minutes.

产品信息
部件	milk-based - (1 container, 50 mL). Antibody Stripping Solution (10x) - (1 container, 50 mL). Blocking Buffer (20x)
检测方法	Chemiluminescence

应用
主要应用	Western Blotting
应用说明	The MILLIPORE Re-Blot Western Blot Recycling Kit is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site. The Re-Blot Western Blot Recycling Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen. This product is for research use only; not for diagnostic or in vivo use.

应用

主要应用

Western Blotting

应用说明

The MILLIPORE Re-Blot Western Blot Recycling Kit is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site.

The Re-Blot Western Blot Recycling Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

This product is for research use only; not for diagnostic or in vivo use.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Kit components should be stored at 2-8° C upon arrival. Product is stable for 3 to 6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37° C before use.