merck millipore,默克密理愽
  • merck millipore,默克密理博,04-1525,Anti-TAFII250 Antibody, clone 6B3

  • merck millipore,默克密理博,04-1525,Anti-TAFII250 Antibody, clone 6B3

    产品名称：Anti-TAFII250 Antibody, clone 6B3
    产品型号：04-1525
    Anti-TAFII250 Antibody, clone 6B3 is a Mouse Monoclonal Antibody for detection of TAFII250 also known as Cell cycle gene 1 protein & has been validated in WB, ICC, IHC & IP.

    merck millipore,默克密理博,04-1525,Anti-TAFII250 Antibody, clone 6B3

  • 产品介绍
  • merck millipore,默克密理博,04-1525,Anti-TAFII250 Antibody, clone 6B3

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HIHC, WB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 04-1525
    描述 Anti-TAFII250 Antibody, clone 6B3
    Alternate Names
    • complementation of cell cycle block, G1-to-S 2 <br />transcription factor TFIID p250 polypeptide
    • Cell cycle gene 1 protein
    • TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
    • TAF1 RNA polymerase II, TATA box binding protein-associated factor, 250kDa
    • TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 250kD
    • TBP-associated factor 1
    • TBP-associated factor 250 kDa
    • Transcription initiation factor TFIID 250 kDa subunit
    • cell cycle, G1 phase defect
    背景信息 TATA-binding protein associated factor 250 (TAFII250) is the largest subunit of the transcription factor TFIID. Studies have shown that TAFII250 has serine/threonine domains that can autophosphorylate and transphosphorylate the large subunit of TFIIF. More recent studies have shown that TAFII250 is also a histone acetyltransferase, suggesting that transcriptional co-activators may also have a role in targeting acetylation activity to promoters.
    产品信息
    格式 Purified
    控制
    • HeLa cell lysate
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
    应用
    应用 Anti-TAFII250 Antibody, clone 6B3 is a Mouse Monoclonal Antibody for detection of TAFII250 also known as Cell cycle gene 1 protein & has been validated in WB, ICC, IHC & IP.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunoprecipitation
    Cited by external research group using a representative lot. See Hilton, H.L., et. al (2005) Mol. Cell. Bio. 25(10):4321–4332.
    生物信息
    免疫原品种 Human TAFII250
    表位 Unknown
    克隆 6B3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes TAFII250.
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_004597.2
    Entrez基因汇总 Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.[provided by RefSeq].
    基因符号
    • p250
    • TAF1
    • BA2R
    • CCG1
    • CCGS
    • DYT3
    • KAT4
    • N-TAF1
    • NSCL2
    • OF
    • P250
    • TAF(II)250
    • TAF2A
    • TAFII-250
    • TAFII250
    纯化方法 Protein G purfied
    UniProt编号
    • P21675
    UniProt汇总 FUNCTION: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.

    CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

    COFACTOR: Magnesium.

    ENZYME REGULATION: Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1.

    SUBUNIT STRUCTURE: TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts with SV40 Large T antigen.

    SUBCELLULAR LOCATION: Nucleus

    PTM: Phosphorylated by casein kinase II in vitro.

    INVOLVEMENT IN DISEASE: Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.

    SEQUENCE SIMILARITIES: Belongs to the TAF1 family.

    Contains 2 bromo domains.

    Contains 1 HMG box DNA-binding domain.

    Contains 2 protein kinase domains.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa cell lysate.

    Western Blot Analysis: 1 µg/ml of this antibody detected TAFII250 on 10 µg of HeLa cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,04-1525,Anti-TAFII250 Antibody, clone 6B3

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