merck millipore,默克密理博,AB1607,Anti-Phosphothreonine Antibody

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merck millipore,默克密理博,AB1607,Anti-Phosphothreonine Antibody
产品名称：Anti-Phosphothreonine Antibody
产品型号：AB1607
Anti-Phosphothreonine Antibody is an antibody against Phosphothreonine for use in ELISA, IH(P), IP & WB.

merck millipore,默克密理博,AB1607,Anti-Phosphothreonine Antibody

产品介绍

merck millipore,默克密理博,AB1607,Anti-Phosphothreonine Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
A	ELISA, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB1607
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Phosphothreonine Antibody
背景信息	Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.

产品信息
格式	Affinity Purified
控制	Mouse brain lysate.
演示	Purified rabbit polyclonal in PBS buffer, containing 50% Glycerol, 0.01% NaN3.

应用
应用	Anti-Phosphothreonine Antibody is an antibody against Phosphothreonine for use in ELISA, IH(P), IP & WB.
主要应用	ELISA Western Blotting
应用说明	ELISA (kinase assay): A 0.5 μg/mL concentration of a previous lot was used in ELISA. Immunoprecipitation: A previous lot of this antibody was used in IP. Immunohistochemistry (paraffin): Representative image from a previous lot. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Keyhole Limpet Hemocyanin (KLH) – conjugated phosphothreonine conjugates and Phosvitin.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	By direct competitive ELISA methodology (antigen capture) the specificity of this antibody has been determined to be phosphothreonine. Binding of Ly-pT-Gly-HRP (0.2μg/100μL/well) to an anti-phosphothreonine coated microplate (0.5μg/100μL/well) is inhibited by 95% with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine, or threonine at concentrations of up to 1 mg/mL. Antibody AB1607 recognizes phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins.
品种反应性	All
Species Reactivity Note	Anti-phosphothreonine is species independent
抗体类型	Polyclonal Antibody
修改	Phosphorylation
纯化方法	Immunoaffinity purified

产品使用声明
质量保证	Routinely evaluated by Western Blot on Calyculin/Okadaic Acid treated HEK293 lysate. Western Blot Analysis: 1:500 dilution of this lot detected PHOSPHOTHREONINE on 10 μg of Calyculin/Okadaic Acid treated HEK293 lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.