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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1603 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Phosphoserine Antibody 背景信息 Cellular responses to a variety of extracellular signals occur through phosphorylation or dephosphorylation of intracellular proteins. Abnormal protein phosphorylation may be involved in the progression of numerous diseases, including many forms of cancer, immune system dysfunction and cardiovascular disease. Such changes in protein phosphorylation may be detected by immunocytochemical mapping of cells labeled with anti-phosphoserine or anti-phosphothreonine antibodies and can be correlated with cellular, electrophysiological or behavioral state changes. 产品信息 格式 Affinity Purified 控制 - NIH 3T3 cells (+/- TPA), K562 cells, and EGF-stimulated A431 cells.
演示 Purified rabbit in buffer containing 50% glycerol. 应用 应用 Anti-Phosphoserine Antibody detects level of Phosphoserine & has been published & validated for use in ELISA, IH, IP & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Immunoprecipitation (tissue extracts):
10-20 μg anti-pS/mg protein was used from a previous lot.
Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 μg/500 μL of extracts. Optimal concentrations must be determined experimentally.
ELISA (kinase assay):
A previous lot of this anitbody was used at a 1:250-1:500 dilution.
Immunohistochemistry:
A previous lot of this antibody was used at a 1:50 dilution.
As the antibody detects all phosphoserines, any phosphorylated serine protein or peptide can be used to block antibody staining.
Typically a 10 M excess of peptide is used in Tris based buffers.
Western Blot Analysis:
1:500 dilution of a previous lot
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Keyhole Limpet Hemocyanin(KLH)- conjugated phosphoserine conjugates. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins. Does not cross react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross reactivity with free phosphothreonine. Readily reacts with known phosphoproteins such as phosvitin and alpha casein. Anti-phosphoserine is species independent in reactivity 品种反应性 All Species Reactivity Note Anti-phosphoserine is species independent. 抗体类型 Polyclonal Antibody 修改 - Phosphorylation
纯化方法 Phosphoserine affinity chromatography 产品使用声明 质量保证 Routinely evaluated by Western Blot on NGF treated PC12 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected serine phosphorylated proteins on 10 μg of NGF treated PC12 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for up to 6 months at -20°C in undiluted aliquots from date of receipt. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,420112,JAK3 Inhibitor, Negative Control - CAS 153437-07-1 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,23-026M,SCF-TrCP1 Protein Complex, Active, 250 µg
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