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merck millipore,默克密理博,05-602,Anti-LEF Antibody, clone 2D12
产品名称：Anti-LEF Antibody, clone 2D12
产品型号：05-602
Anti-LEF Antibody, clone 2D12 is a Mouse Monoclonal Antibody for detection of LEF also known as lymphoid enhancer-binding factor & has been tested in WB.
merck millipore,默克密理博,05-602,Anti-LEF Antibody, clone 2D12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-602 品牌系列 Upstate
商名 - Upstate
描述 Anti-LEF Antibody, clone 2D12 背景信息 Monoclonal Anti-LEF-1 (β-Catenin Binding Domain) recognizes the β-Catenin Binding Domain of human LEF-1 using immunoblotting (55-57 kDa). Lymphoid enhancer factor, LEF-1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. 产品信息 格式 Purified 控制 - Jurkat nuclear extract
演示 100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL. Store at -20°C. 应用 应用 Anti-LEF Antibody, clone 2D12 is a Mouse Monoclonal Antibody for detection of LEF also known as lymphoid enhancer-binding factor & has been tested in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Residues 1-85 of human Lymphoid Enhancer Factor (LEF) 克隆 clone 2D12 宿主 Mouse 特异性 LEF that appears as multiple polypeptides 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_016269
Entrez基因汇总 Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM] 基因符号 - DKFZp586H0919
- LEF1
- LEF-1
- TCF1-alpha
- TCF1ALPHA
纯化方法 Thiophilic and size exclusion chromatography UniProt编号 - Q9UJU2
UniProt汇总 FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
SIZE: 399 amino acids; 44201 Da
SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.产品使用声明 质量保证 routinely evaluated by immunoblot on Jurkat cell lysate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB8905-K,Anti-Measles Blend, hemagglutinin, clones CV1, CV4 (1mg)
下一件merck millipore产品：merck millipore,默克密理博,MABS278,Anti-EMAPII Antibody, clone M7/1, (Preservat ive free)
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