merck millipore,默克密理愽
  • merck millipore,默克密理博,AB1576,Anti-Cysteine String Protein Antibody

  • merck millipore,默克密理博,AB1576,Anti-Cysteine String Protein Antibody

    产品名称：Anti-Cysteine String Protein Antibody
    产品型号：AB1576
    This Anti-Cysteine String Protein Antibody is validated for use in WB, IC, IH for the detection of Cysteine String Protein.

    merck millipore,默克密理博,AB1576,Anti-Cysteine String Protein Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB1576,Anti-Cysteine String Protein Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    AWBRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB1576
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Cysteine String Protein Antibody
    Alternate Names
    • DnaJ Homologue Subfamily C member 5
    • CSP
    背景信息 Cysteine-string proteins are membrane-bound proteins associated primarily with nerve endings and synaptic vesicles in the brain where they serve an important role in presynaptic function and may be involved in calcium-dependent neurotransmitter release at nerve endings. CSPs posses a strech of amino acids highly enriched in cysteine residues that are post-translationally palmitoylated. CSPs may function as homodimers.
    产品信息
    格式 Serum
    控制
    • POSITIVE CONTROL: brain samples
    演示 Liquid rabbit serum containing no preservatives
    应用
    应用 This Anti-Cysteine String Protein Antibody is validated for use in WB, IC, IH for the detection of Cysteine String Protein.
    主要应用
    • Western Blotting
    应用说明 Immunohistochemistry on formalin fixed tissue: 1:1,000. (Recommend Cat. No. AB1576P).Immunocytochemistry on PC-12 cells: 1:1,000. (Recommend Cat. No. AB1576P).Immunoblotting: 1:5,000. CSPs should be resolved on 12-15% SDS-PAGE gels under NON-REDUCING conditions. CSPs typically migrate as 33-35 kDa species in vertebrate samples (dimers may also be seen). If one must perform SDS-PAGE under reducing conditions, it is necessary to alkylate the CSPs to prevent smearing.To ensure proper identification of CSPs, samples may be deacylated by treatment with 1M neutral hydroxylamine or 0.1M KOH in methanol resulting in a 6-7 kDa shift in electrophoretic mobility of the CSP band. Deacylation will usually result imn some loss of reactivity on western blots so sufficient protein should be loaded. Alternatively, because all CSPs appear to be acidic (approximate pI of 4.6-4.8) 2-D gel electrophoresis may be usefull to distinguish some CSPs. Finally, some sample treatments may cause partial deacylation of CSPs resulting in a smear of immunoreactivity below the mass of the fatty acylated monomer.Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Recombinant rat cysteine-string protein with the cysteine string deleted.
    宿主 Rabbit
    特异性 Cysteine-string Protein. The antibody recognizes primarily the alpha isoform of CSP with some recognition of the beta isoforms. Slight reactivity with the gamma isoforms may be detected.
    品种反应性 All
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_025219.1
    基因符号
    • FLJ00118
    • DNAJC5
    • CSP
    • DKFZP434N1429
    • FLJ13070
    • DKFZp761N1221
    UniProt编号
    • Q9H3Z4
    UniProt汇总 FUNCTION: SwissProt: Q9H3Z4 # May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).
    SIZE: 198 amino acids; 22149 Da
    SUBCELLULAR LOCATION: Membrane; Lipid-anchor (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    TISSUE SPECIFICITY: Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
    PTM: Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity).
    SIMILARITY: SwissProt: Q9H3Z4 ## Contains 1 J domain.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB1576,Anti-Cysteine String Protein Antibody

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