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  • merck millipore,默克密理博,AB1511,Anti-Glutamate Decarboxylase 65 & 67 Antibody

  • merck millipore,默克密理博,AB1511,Anti-Glutamate Decarboxylase 65 & 67 Antibody

    产品名称：Anti-Glutamate Decarboxylase 65 & 67 Antibody
    产品型号：AB1511
    This Anti-Glutamate Decarboxylase 65 & 67 Antibody is validated for use in IH, WB for the detection of Glutamate Decarboxylase 65 & 67.

    merck millipore,默克密理博,AB1511,Anti-Glutamate Decarboxylase 65 & 67 Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB1511,Anti-Glutamate Decarboxylase 65 & 67 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Fe, H, R, MWB, IHCRbPurifiedPolyclonal Antibody
    描述
    产品目录编号 AB1511
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Glutamate Decarboxylase 65 & 67 Antibody
    Alternate Names
    • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
    • 65 kDa glutamic acid decarboxylase
    • Glutamate decarboxylase 65 kDa isoform
    • Glutamate decarboxylase-2 (pancreas)
    • glutamate decarboxylase 2
    • glutamate decarboxylase 2 (pancreatic islets and brain,65kD)
    • glutamate decarboxylase 2 (pancreatic islets and brain
    背景信息 Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
    产品信息
    格式 Purified
    控制
    • Whole rat brain lysate, mouse brain cell extract.
    演示 Purified rabbit polyclonal in buffer containing PBS-BSA containing 0.1% sodium azide.
    应用
    应用 This Anti-Glutamate Decarboxylase 65 & 67 Antibody is validated for use in IH, WB for the detection of Glutamate Decarboxylase 65 & 67.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Immunohistochemistry:
    1:200-1:5,000 dilution of a previous lot was used in immunohistochemistry (overnight PAP or ABC). Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily. Also reactive on paraffin embedded tissue. Protease digestion is not required.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 A synthetic peptide with the amino acid sequence [C]DFLIEEIERLGQDL from rat glutamate decarboxylase (GAD65; C-terminus residues [Cys] + 572-585) (1).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Glutamate Decarboxylase (GAD65 and GAD67).
    Western blot reveals a doublet at approximately 65/68 kDa.
    Immunohistochemical staining can be abolished by preincubation with 1-10 μg peptide (Catalog Number AG252) per mL of diluted antibody.
    品种反应性 FelineHumanRatMouse
    Species Reactivity Note Human, mouse, rat, and feline.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_000809
    Entrez基因汇总 This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.
    基因符号
    • GAD-65
    • GAD2
    • MGC161607
    • GAD65
    • MGC161605
    纯化方法 Ammonium sulfate precipitation
    UniProt编号
    • Q05329
    UniProt汇总 FUNCTION: SwissProt: Q05329 # Catalyzes the production of GABA.
    COFACTOR: Pyridoxal phosphate.
    SIZE: 585 amino acids; 65411 Da
    SUBUNIT: Homodimer (By similarity).
    SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
    PTM: Phosphorylated; which does not affect kinetic parameters or subcellular location. & Palmitoylated; which is required for presynaptic clustering.
    SIMILARITY: SwissProt: Q05329 ## Belongs to the group II decarboxylase family.
    产品使用声明
    质量保证 Evaluated by Western Blot on mouse brain lysates.

    Western Blot Analysis:
    1:500 dilution of this antibody detected GAD 65&67 on 10 μg of mouse brain lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB1511,Anti-Glutamate Decarboxylase 65 & 67 Antibody

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