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merck millipore,默克密理博,09-868,Anti-PHD finger protein 8 Antibody
产品名称：Anti-PHD finger protein 8 Antibody
产品型号：09-868
Anti-PHD finger protein 8 Antibody is a Rabbit Polyclonal Antibody for detection of PHD finger protein 8 also known as Histone lysine demethylase PHF8, PHD finger protein 8 & has been validated in WB, More>>
merck millipore,默克密理博,09-868,Anti-PHD finger protein 8 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, M, R, Chp, B, Rb, Rhesus Macaque ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-868 描述 Anti-PHD finger protein 8 Antibody Alternate Names - PHD finger protein 8
- Histone lysine demethylase PHF8
背景信息 PHF8 (PHD finger protein 8) is a histone lysine demethylase. This protein preferentially demethylates histones that are monomethylated or dimethylated at lysine residues. Activity of this protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen. PHF8 is a member of the jumonji family and contains a jumonji C (JmjC) domain and a PHD (plant homeodomain) zinc-finger. Members of the jumonji protein family are known to play a role a chromatin remodeling and histone demethylation. Defects in PHF8 are the cause of mental retardation syndromic X-linked Siderius type. A disorder characterized by mild to borderline mental retardation with or without cleft lip/cleft palate. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-PHD finger protein 8 Antibody is a Rabbit Polyclonal Antibody for detection of PHD finger protein 8 also known as Histone lysine demethylase PHF8, PHD finger protein 8 & has been validated in WB, ICC & IP. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PHD finger protein 8 in NIH/3T3, A431, and HeLa cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human PHD finger protein 8. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 CanineHumanMouseRatChimpanzeeBovineRabbitRhesus Macaque Species Reactivity Note Demonstrated to react with Human and Mouse. Predicted to react with Rat, Chimpanzee, Bovine, Rabbit, Rhesus Monkey, Canine, and Porcine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001171825
Entrez基因汇总 The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - ZNF422
- PHF8
纯化方法 Affinity purified UniProt编号 - Q9UPP1
UniProt汇总 FUNCTION: Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
CATALYTIC ACTIVITY: Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2.
Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2.
COFACTOR: Binds 1 Fe2+ ion per subunit Probable.
SUBUNIT STRUCTURE: Interacts with POLR1B, UBTF, SETD1A, HCFC1, E2F1 and ZNF711.
SUBCELLULAR LOCATION: Nucleus. Nucleus › nucleolus. Note: Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis.
DOMAIN: The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 promotes its access to H3K9me2.
The linker region is a critical determinant of demethylase specificity. It enables the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3.
PTM: Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.
INVOLVEMENT IN DISEASE: Defects in PHF8 are the cause of mental retardation syndromic X-linked Siderius type (MRXSSD) [MIM:300263]. A disorder characterized by mild to borderline mental retardation with or without cleft lip/cleft palate.
SEQUENCE SIMLARITIES: Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:
KM=134 µM for histone H3 H3K9Me2 Ref.23
KM=8 µM for histone H3 H3K4me3 and H3K9Me2
SEQUENCE CAUTION: The sequence BAA83063.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence BAB13877.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence CAI41577.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI41581.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI41582.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI42861.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI45929.1 differs from that shown. Reason: Erroneous termination at position 419. Translated as Arg.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1:333 dilution of this antibody detected PHD finger protein 8 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µL
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