- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,AB144P,Anti-Choline Acetyltransferase Antibody
产品名称：Anti-Choline Acetyltransferase Antibody
产品型号：AB144P
Anti-Choline Acetyltransferase Antibody detects level of ChAT and has been published and validated for use in IH(P), IC, IH and WB.
merck millipore,默克密理博,AB144P,Anti-Choline Acetyltransferase Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Zebrafish, H, R, M, Mk, Op, Av, Ch, Gp WB, ICC, IHC Gt Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB144P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Choline Acetyltransferase Antibody 概述 Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Alternate Names - ChAT
- Choline acetylase
- acetyl CoA:choline O-acetyltransferase
- choline acetyltransferase
背景信息 Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. 产品信息 格式 Affinity Purified 控制 - Human placenta lysates, rat forebrain/rostral hypothalamus.
演示 Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide. 应用 应用 Anti-Choline Acetyltransferase Antibody detects level of ChAT and has been published and validated for use in IH(P), IC, IH and WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.生物信息 免疫原品种 Human placental enzyme. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Goat 特异性 Choline acetyltransferase found in cholinergic neurons in brain and spinal cord. 品种反应性 ZebrafishHumanRatMouseMonkeyOpossumAvianChickenGuinea Pig Species Reactivity Note Human and mouse. Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).抗体类型 Polyclonal Antibody Entrez基因编号 - NM_020549.3
Entrez基因汇总 Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. 基因符号 - CMS1A2
- CHAT
- ChAT
- CHOACTase
- CMS1A
纯化方法 Affinity Purfied UniProt编号 - P28329
UniProt汇总 FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.产品使用声明 质量保证 Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.包装信息 数量 500 µL
上一件merck millipore产品：merck millipore,默克密理博,AB1858,Anti-Osteonectin Antibody
下一件merck millipore产品：merck millipore,默克密理博,208902,Calpastatin Peptide - Calbiochem
相关产品
merck millipore,默克密理博,219384,Anti-Cathepsin S Rabbit pAb
merck millipore,默克密理博,118415,ADP-HPD, Dihydrate, Ammonium Salt - Calbiochem
merck millipore,默克密理博,04-321,Anti-4E-BP1 Antibody, rabbit monoclonal
merck millipore,默克密理博,AB1858,Anti-Osteonectin Antibody
merck millipore,默克密理博,208902,Calpastatin Peptide - Calbiochem
merck millipore,默克密理博,07-1437,Anti-PDGF-BB Antibody
merck millipore,默克密理博,04-486,Anti-RhoG Antibody, clone 1F3 B3 E5
merck millipore,默克密理博,MAB80122,Anti-Human Metapneumovirus Antibody, clone 67
merck millipore,默克密理博,178461,Apolipoprotein C-III, Human Plasma, Very Low-Density Lipoprotein