merck millipore,默克密理博,07-1437,Anti-PDGF-BB Antibody

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merck millipore,默克密理博,07-1437,Anti-PDGF-BB Antibody
产品名称：Anti-PDGF-BB Antibody
产品型号：07-1437
Anti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.

merck millipore,默克密理博,07-1437,Anti-PDGF-BB Antibody

产品介绍

merck millipore,默克密理博,07-1437,Anti-PDGF-BB Antibody

Replacement Information
Replacement Information	07-1437 is a recommended replacement for AB1487P.

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ELISA, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1437
Replaces	AB1487P
描述	Anti-PDGF-BB Antibody
Alternate Names	V-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog) Platelet-derived growth factor B chain precursor Platelet-derived growth factor beta polypeptide Platelet-derived growth factor, beta polypeptide (oncogene SIS) oncogene SIS Platelet-derived growth factor Platelet-derived growth factor beta Platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) Platelet-derived growth factor, B chain
背景信息	Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.

产品信息
格式	Affinity Purified
演示	Purified rabbit polyclonal IgG lyophilized from 0.5X PBS, pH 7.4. Reconstitute with sterile distilled water.

应用
应用	Anti-PDGF-BB Antibody detects level of PDGF-BB & has been published & validated for use in WB, ELISA, NEUT.
主要应用	ELISA Western Blotting
应用说明	ELISA: 0.5 μg/mL dilution of a previous lot will allow detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-BB is used to coat the plate. Neutralization: To yield ½ maximal inhibition (ND50) of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of a previous lot of this antibody was required. Immunohistochemistry(paraffin): Anti-IGF-1R, representative staining-pattern and morphology on ductal breast carcinoma. Tissue was pretreated with EDTA, pH 8.0 for Epitope retrieval. A previous lot of this antibody was diluted to 1:200 (RT incubation), using IHC-Select® Detection with HRP-DAB. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Highly purified (>98%) recombinant human PDGF-BB
宿主	Rabbit
特异性	Reacts with human PDGF-BB.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002608
Entrez基因汇总	The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. [provided by RefSeq].
基因符号	Becaplermin PDGFB FLJ12858 PDGF2 c-sis PDGF-2 SSV SIS
纯化方法	Affinity Purfied
UniProt编号	P01127
UniProt汇总	FUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with XLKD1 (By similarity). SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. DISEASE: A chromosomal aberration involving PDGFB is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with COLA1. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities. PHARMACEUTICAL: Available under the name Regranex (Ortho-McNeil). Used to promote healing in diabetic neuropathic foot ulcers. MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. SIMILARITY: Belongs to the PDGF/VEGF growth factor family.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Huvec lysates. Western Blot Analysis: 1:500 dilution of this lot detected PDGF-BB on 10 μg of Huvec lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

储存和货运信息

存储条件

Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.