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描述 概述 The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.
pIEx-5 encodes the signal peptide of adipokinetic hormone (AKH) to allow secretion of the expressed protein, and C-terminal S•Tag and His•Tag coding sequences. For sequencing or PCR of recombinants in pIEx-5, refer to the table below to choose the appropriate primers.Vector 5' Primer 3' Primer alt 5' Primer pIEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-3 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer pIEx-4 IE1 Promoter Primer AS S•Tag 18mer Primer NA pIEx-5 IE1 Promoter Primer AS S•Tag 18mer Primer NA pBiEx-1 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter PrimerpBiEx-2 S•Tag 18mer Primer AS HSV•Tag Primer IE1 Promoter Primer
T7 Promoter PrimerpBiEx-3 IE1 Promoter Primer AS S•Tag 18mer Primer T7 Promoter Primer 产品目录编号 71242 品牌系列 Novagen®
同义词 InsectDirect™ System- pIEx™-5 DNA 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 -20°C
无需冷冻 No
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