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检测方法 Colorimetric 描述 概述 Assay kit useful for the rapid and accurate measurement of nitric oxide synthase (NOS) activity. This colorimetric assay uses lactate dehydrogenase (LDH) to destroy excess NADPH (cofactor for NOS) which interferes with the chemistry of Griess Reagent, commonly used for nitrite/nitrate detection. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI. 产品目录编号 482702 品牌系列 Calbiochem®
同义词 NOS Assay Kit, Colorimetric 参考 参考 Nims, R.W., et al. 1995. Methods Enzymol. 7, 48.
Moncada, S. 1992. Acta Physiol. Scand. 145, 201.
Nathan, C. 1992. FASEB J. 6, 3051.
Green, L.C., et al. 1982. Anal. Biochem. 126, 131.产品信息 检测方法 Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 Assay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol. 生物信息 化验时间 2 h 样品类型 Plasma, serum, urine, tissue culture medium, and tissue homogenates 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 -20°C
存储条件 Upon arrival store the entire contents of the kit at -20°C. 无需冷冻 No
补充信息 试剂盒包含 Assay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol.
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